Coding

Part:BBa_K4645002:Design

Designed by: Ruixuan Zhu   Group: iGEM23_HZAU-China   (2023-10-02)


NeuB: scFv ( single-chain fragment variable）that inhibits the immune response to the feline


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal XhoI site found at 276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the basis of the expression of functional target proteins, His tag is added, which can help us better obtain high-purity target proteins. Additionally, an OmpA signaling peptide was fused to the N-terminus of NeuB to aid in its secretion to the periplasmic space for efficient antigen neutralization.


Source

Velocimmune mice were immunized with recombinant dimeric Fel d 1 and produced polyclonal antibodies. The amino acid sequence of one of the most effective monoclonal antibodies REGN1909 was obtained through literature review.

References